Research Article
Selection of Suitable Reference Genes for qPCR Gene Expression Analysis of HepG2 and L02 in Four Different Liver Cell Injured Models
Table 1
Details of the ten candidate reference genes and primers used in the qPCR.
| | Gene | Description | Primer: forward/reverse(5-3) | Length (bp) | Accession number |
| | ACTB | β-Actin | F: AAGGCCAACCGCGAGAAGAT
R: GCCAGAGGCGTACAGGGATA | 102 | NM_001101 | | B2M | Beta-2 microglobulin | F: GTTTACTCACGTCATCCAGC
R:AGACAAGTCTGAATGCTCCA | 141 | NM_004048 | | GAPDH | Glyceraldehade-3-phosphate dehydrogenase | F: GCCTCCTGCACCACCAACTG
R: CCATCACGCCACAGTTTCCC | 149 | NM_002046 | | TUBB2a | Tubulin beta 2a | F: AACGCCACCCTCTCTGTCCA
R: GCCGACACCAGGTGGTTGAG | 143 | NM_001069 | | HPRT1 | Hypoxanthine phosphoribosyltransferase 1 | F: ACTGAACGTCTTGCTCGAGA
R: TGATGTAATCCAGCAGGTCA | 112 | NM_000194 | | SDHA | Succinate dehydrogenase complex flavoprotein subunit A | F: AAAGATCACGTCTACCTGCA
R: CATGTTATAATGCACGGTGG | 150 | NM_004168 | | TBP | TATA-box binding protein | F: GTTCAGCAGTCAACGTCCCA
R: TCATGGGGGAGGGATACAGT | 127 | NM_003194 | | YWHAZ | Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta | F: CAGGCTGAGCGATATGATGA
R: CCTACGGGCTCCTACAACAT | 126 | NM_003406 | | CYC1 | Cytochrome c isoform 1 | F: CCAAAACCATACCCCAACAG
R: AGTCCTCACCACCATGCCTA | 103 | NM_001916 | | GUSB | Glucuronidase beta | F: GTTCCTTTTGCGAGAGAGAT
R: ACACGCAGGTGGTATCAGTC | 124 | NM_000181 |
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