Research Article
Selection of Suitable Reference Genes for qPCR Gene Expression Analysis of HepG2 and L02 in Four Different Liver Cell Injured Models
Table 3
Expression stability values of the reference genes calculated by BestKeeper in HepG2.
| | Rank | WT | EtOH
100 mM | EtOH
200 mM | EtOH
400 mM | H2O2
200 μM | H2O2
400 μM | H2O2
800 μM | APAP
2.5 mM | APAP
5 mM | APAP
10 mM | CCl4
0.1% | CCl4
0.2% | CCl4
0.4% |
| | 1 | GUSB
| HPRT1
| SDHA
| B2M
| CYC1
| CYC1
| TBP
| SDHA
| CYC1
| GUSB
| GUSB
| HPRT1
| TBP
| | 2 | ACTB
| CYC1
| TBP
| TBP
| TUBB2a
| YWHAZ
| CYC1
| TBP
| GUSB
| CYC1
| CYC1
| GUSB
| SDHA
| | 3 | TUBB2a
| TBP
| CYC1
| SDHA
| SDHA
| GUSB
| B2M
| TUBB2a
| TUBB2a
| TUBB2a
| HPRT1
| TBP
| CYC1
| | 4 | CYC1
| SDHA
| GUSB
| YWHAZ
| TBP
| HPRT1
| SDHA
| GUSB
| HPRT1
| ACTB
| B2M
| CYC1
| TUBB2a
| | 5 | B2M
| TUBB2a
| ACTB
| HPRT1
| ACTB
| TUBB2a
| HPRT1
| YWHAZ
| B2M
| TBP
| TBP
| YWHAZ
| HPRT1
| | 6 | TBP
| ACTB
| B2M
| CYC1
| B2M
| TBP
| GAPDH
| HPRT1
| YWHAZ
| B2M
| SDHA
| TUBB2a
| GUSB
| | 7 | HPRT1
| YWHAZ
| TUBB2a
| TUBB2a
| GUSB
| ACTB
| GUSB
| CYC1
| ACTB
| HPRT1
| YWHAZ
| ACTB
| YWHAZ
| | 8 | SDHA
| GUSB
| GAPDH
| GUSB
| YWHAZ
| B2M
| TUBB2a
| ACTB
| TBP
| SDHA
| TUBB2a
| B2M
| ACTB
| | 9 | YWHAZ
| B2M
| HPRT1
| ACTB
| HPRT1
| SDHA
| ACTB
| B2M
| SDHA
| GAPDH
| ACTB
| SDHA
| B2M
| | 10 | GAPDH
| GAPDH
| YWHAZ
| GAPDH
| GAPDH
| GAPDH
| YWHAZ
| GAPDH
| GAPDH
| YWHAZ
| GAPDH
| GAPDH
| GAPDH
|
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