Bioinformatical Analysis of miRNA-mRNA Interaction Network Underlying Macrophage Aging and Cholesterol-Responsive Difference between Young and Aged Macrophages

Table 1

The top 9 Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathways of the differentially expressed miRNAs targets between aged and young macrophages.

Category	GO term	Description	Count	Genes
BP	GO:0006955	Immune response	16	CCL24, CCL2, CXCR5, CXCL5, ENPP2, PRG4, CXCL13, H2-OB, MCPT4, CCL8, CMA1, TGTP2, CCL5, LTB, CCL7, BMPR1A
	GO:0006954	Inflammatory response	10	CCL24, SELP, CCL2, CXCL5, CXCL13, EPHX2, CCL8, CD5L, CCL5, CCL7
	GO:0006935	Chemotaxis	9	CCL24, CCL2, CXCR5, CXCL5, CXCL13, ENPP2, CCL8, CCL5, CCL7
	GO:0045766	Positive regulation of angiogenesis	8	CCL24, PTGIS, CYP1B1, LRG1, SFRP2, HSPB1, CMA1, CCL5
	GO:0055114	Oxidation-reduction process	8	CYP7B1, PTGIS, CYP1B1, SCD2, MAOA, CH25H, CP, DHCR24
	GO:0070098	Chemokine-mediated signaling pathway	7	CCL24, CCL2, CXCL5, CXCL13, CCL8, CCL5, CCL7
	GO:0071347	Cellular response to interleukin-1	7	LCN2, CCL24, CCL2, PTGIS, CCL8, CCL5, CCL7
	GO:0006629	Lipid metabolic process	7	CYP7B1, PTGIS, SCD2, ENPP2, CH25H, EPHX2, DHCR24
	GO:0008284	Positive regulation of cell proliferation	7	PRL2C3, CCND2, ENPP2, SFRP2, MZB1, PLAC8, TIMP1
MF	GO:0005125	Cytokine activity	10	CCL24, CCL2, CXCR5, CXCL5, ENPP2, PRG4, CXCL13, H2-OB, MCPT4, CCL8, CMA1, TGTP2, CCL5, LTB, CCL7, BMPR1A
	GO:0008009	Chemokine activity	7	CCL24, SELP, CCL2, CXCL5, CXCL13, EPHX2, CCL8, CD5L, CCL5, CCL7
	GO:0005525	GTP binding	7	CCL24, CCL2, CXCR5, CXCL5, CXCL13, ENPP2, CCL8, CCL5, CCL7
	GO:0016491	Oxidoreductase activity	7	CCL24, PTGIS, CYP1B1, LRG1, SFRP2, HSPB1, CMA1, CCL5
	GO:0042803	Protein homodimerization activity	7	CYP7B1, PTGIS, CYP1B1, SCD2, MAOA, CH25H, CP, DHCR24
	GO:0008201	Heparin binding	6	CCL24, CCL2, CXCL5, CXCL13, CCL8, CCL5, CCL7
	GO:0005506	Iron ion binding	6	LCN2, CCL24, CCL2, PTGIS, CCL8, CCL5, CCL7
	GO:0004497	Monooxygenase activity	4	CYP7B1, PTGIS, SCD2, ENPP2, CH25H, EPHX2, DHCR24
	GO:0030414	Peptidase inhibitor activity	4	PRL2C3, CCND2, ENPP2, SFRP2, MZB1, PLAC8, TIMP1
CC	GO:0005615	Extracellular space	29	GDF3, CCL2, CXCL5, ENPP2, LUM, IGFBP7, SERPINB1A, CCL8, CCL5, MMP3, CCL7, TIMP1, PRL2C3, CCL24, PTGIS, LRG1, MS4A1, CPA3, LTB, SELP, ACTA2, PRG4, SERPING1, LCN2, CXCL13, SFRP2, SERPINB2, HSPB1, CP
	GO:0005576	Extracellular region	25	GDF3, CCL2, CXCL5, ENPP2, LUM, IGFBP7, CCL8, CCL5, MMP3, CCL7, TIMP1, CCL24, PRL2C3, PRG4, MZB1, SERPING1, CD5L, LCN2, BGN, PENK, CXCL13, SFRP2, SERPINB2, CMA1, CP
	GO:0070062	Extracellular exosome	21	CPNE8, ACTA2, LUM, IGFBP7, EPHX2, SERPINB1A, SERPING1, CD5L, TIMP1, LCN2, CD38, CD55, ASPA, CD19, BGN, LRG1, MS4A1, HSPB1, CD79B, CP, VSIG4
	GO:0009897	External side of plasma membrane	11	LY6A, FCER1A, LY6C1, SELP, CD55, CD19, CXCR5, MS4A1, CD79B, CD79A, BMPR1A
	GO:0005789	Endoplasmic reticulum membrane	8	CYP7B1, PTGIS, CYP1B1, SCD2, CH25H, TGTP2, DHCR24, GIMAP1
	GO:0031012	Extracellular matrix	7	BGN, LUM, IGFBP7, HSPB1, CMA1, MMP3, TIMP1
	GO:0031225	Anchored component of membrane	4	LY6A, LY6C1, CD55, LY6D
	GO:0031090	Organelle membrane	3	CYP7B1, CYP1B1, SCD2
	GO:0019815	B cell receptor complex	2	CD79B, CD79A
KEGG pathways	mmu04060	Cytokine-cytokine receptor interaction	10	CCL24, CCL2, CXCR5, CXCL5, CXCL13, CCL8, CCL5, LTB, CCL7, BMPR1A
	mmu04062	Chemokine signaling pathway	8	CCL24, CCL2, CXCR5, CXCL5, CXCL13, CCL8, CCL5, CCL7
	mmu05323	Rheumatoid arthritis	6	CCL2, CXCL5, H2-OB, CCL5, MMP3, LTB
	mmu04640	Hematopoietic cell lineage	4	CD38, CD55, CD19, MS4A1
	mmu00380	Tryptophan metabolism	3	KYNU, CYP1B1, MAOA
	mmu04662	B cell receptor signaling pathway	3	CD19, CD79B, CD79A
	mmu00120	Primary bile acid biosynthesis	2	CYP7B1, CH25H

Abbreviations: GO: gene ontology; BP: biological process; MF: molecular functioning; CC: cellular component; KEGG pathways: Kyoto Encyclopedia of Genes and Genomes pathways; GTP: guanosine triphosphate.