BioMed Research International / 2020 / Article / Fig 4

Research Article

Protective Effects of MitoTEMPO on Nonalcoholic Fatty Liver Disease via Regulating Myeloid-Derived Suppressor Cells and Inflammation in Mice

Figure 4

The mRNA and protein levels of liver chronic inflammatory response in mice. (a) The mRNA level of MCP-1 by qRT-PCR assay. mRNA expression was normalized to GAPDH expression and shown as fold change (2-ΔΔCt). (b) The protein level of MCP-1 using immunoblot. The quantification of the intensity of MCP-1 relative to β-actin is shown below. (c, d) The mRNA levels of S100A8 (c) and S100A9 (d) in the liver tissues of each group were measured by qRT-PCR assay. (e, f) Western blot analysis of S100A8 (e) and S100A9 (f) protein expressions in the liver tissues of each group. Relative band density is shown in the bottom. Data are represented as the . HFD vs. lean: , , HFD+Mito vs. HFD: ###P < 0.001, unpaired -test.

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