The Use of RNAi Technology to Interfere with Zfx Gene Increases the Male Rates of Red Deer (Cervus elaphus) Offspring

Figure 2

Transfection interference vector 48 h and 58 h of germ cells. Zfx gene RNAi vector was transferred into red deer germ cells, and the fluorescent labels were transfected germ cells. (a) There was some fluorescence expression at 48 h. (b) Fluorescence expression was the highest at 58 h. The cells were observed under the inversion fluorescence microscope (LeicaDMi8, Leica Microsystems, Wetzlar, Germany). Scale bars represent 100 μm.

(a)

(b)