MicroRNA-148b Inhibits the Malignant Biological Behavior of Melanoma by Reducing Sirtuin 7 Expression Levels

Figure 3

SIRT7 is negatively regulated by miR-148b. A375 cells transfected with miR-148b mimic, miR-148b inhibitor, or empty vector and the level of SIRT7 measured by (a) Western blot and (b) RT-qPCR. TargetScan results of miR-148b (c). The luciferase activity of SIRT7 in the mutant 3UTR of SIRT7 and WT A375 cell after transfection with (d) miR-148b mimic, (e) miR-148b inhibitor, or empty vector. Significant difference at .

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