Phosphatidylinositol-4,5-Biphosphate (PI(4,5)P₂) Is Required for Rapid Endocytosis in Chromaffin Cells

Figure 2

Inhibition of RE by PAO in chromaffin cells subjected to one round of stimulation. Representative whole-cell membrane capacitance (Cm) recordings from cells treated with (a) no drug (control), normal RE is seen following the exocytotic burst; (b) PAO (5 μM); (c) PAO + PI(4,5)P₂ (100 μM); (d) PAO + DTT (0.5 mM). Arrows and bars above traces indicate the timing at which the stimulation protocol was executed to elicit secretion, and the dashed lines are baselines.

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