Establishment and Validation of the Detection of TERT Promoter Mutations by Human Gliomas U251 Cell Lines

Figure 1

Accuracy of the Sanger sequencing assay for TERTp mutations in comparison with the RT-PCR method. Genomic DNA from OL cell lines was used for the detection of TERTp mutations by the RT-PCR method (a) and Sanger sequencing assays (b). OL cell lines presented wild-type TERTp. Genomic DNA from the U251 cell line was used for the detection of TERTp mutations by the RT-PCR method (c) and Sanger sequencing assays (d). U251 cell lines harbored C228T homozygous mutation of the TERTp. The TERTp target gene and internal control were labeled with FAM and ROX probes, respectively. TERTp: the promoter region of the telomerase reverse transcriptase gene.

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