BioMed Research International

Research Article

Establishment and Validation of the Detection of TERT Promoter Mutations by Human Gliomas U251 Cell Lines

Table 1

Primer sequences per gene.
GenesForward primer (5-3)Reverse primer (5-3)Amplified fragments
TERTM13F-AGTGGATTCGCGGGCACAGAM13R-CAGCGCTGCCTGAAACTC235 bp
IDH1M13F-GAGAAGAGGGTTGAGGAGTTM13R-TACCTTGCTTAATGGGTGTA294 bp
IDH2M13F-GCTTGGGGTTCAAATTCTGM13R-CGGTCTGCCACAAAGTCTG343 bp
M13-tagged primerTGTAAAACGACGGCCAGTCAGGAAACAGCTATGACC/
MSP-MTTTCGACGTTCGTAGGTTTTCGCGCACTCTTCCGAAAACGAAACG81 bp
MSP-UTTTGTGTTTTGATGTTTGTAGGTTTTTGTAACTCCACACTCTTCCAAAAACAAAACA93 bp
TERT: telomerase reverse transcriptase; IDH1: isocitrate dehydrogenase 1; IDH2: isocitrate dehydrogenase 2; M13F: M13 universal forward sequence-tagged primers; M13R: M13 universal reverse sequence-tagged primers. MSP-M: methylation special PCR methylated primers; MSP-U: methylation special PCR unmethylated primers.