Research Article
Production and Partial Characterization of α-Amylase Enzyme from Marine Actinomycetes
Table 8
Box-Behnken factorial experimental design for amylase production by Streptomyces rochei HMM 13.
| | Trials | Starch () | K2HPO4 () | pH () | Temp. (°C) () | (U/ml) | (mg/ml) | SA (U/mg) |
| | 1 | 0 | 1 | 0 | 1 | 17.8227 | 10.8877 | 1.63696 | | 2 | 1 | -1 | 0 | 0 | 14.1576 | 63.8529 | 0.22172 | | 3 | 0 | 0 | 1 | -1 | 10.9595 | 13.2233 | 0.82880 | | 4 | 1 | 0 | 0 | 1 | 13.0436 | 12.6484 | 1.03125 | | 5 | 0 | 1 | -1 | 0 | 12.0375 | 45.4552 | 0.26482 | | 6 | 0 | 0 | -1 | 1 | 16.6729 | 12.7562 | 1.30704 | | 7 | 0 | 0 | 1 | 1 | 14.5888 | 71.8300 | 0.20310 | | 8 | 1 | 0 | 1 | 0 | 14.5528 | 99.3906 | 0.14642 | | 9 | -1 | 0 | 1 | 0 | 16.3854 | 37.3703 | 0.43846 | | 10 | 0 | 0 | -1 | -1 | 15.7027 | 12.9358 | 1.21388 | | 11 | 0 | 0 | 0 | 0 | 18.7210 | 71.0754 | 0.26339 | | 12 | 0 | 1 | 0 | -1 | 15.5949 | 11.3548 | 1.37341 | | 13 | -1 | 0 | 0 | 1 | 18.6492 | 41.0354 | 0.45446 | | 14 | 0 | 1 | 1 | 0 | 15.3793 | 68.8835 | 0.22326 | | 15 | 1 | 1 | 0 | 0 | 19.3319 | 8.55205 | 2.26050 | | 16 | 0 | -1 | 0 | -1 | 13.9779 | 13.7982 | 1.01302 | | 17 | 1 | 0 | 0 | -1 | 14.3372 | 18.1461 | 0.79009 | | 18 | -1 | 0 | -1 | 0 | 13.0077 | 12.7562 | 1.01971 | | 19 | -1 | -1 | 0 | 0 | 15.1277 | 24.8297 | 0.60926 | | 20 | -1 | 0 | 0 | -1 | 13.4389 | 14.1935 | 0.94683 | | 21 | 0 | -1 | -1 | 0 | 16.4932 | 29.3572 | 0.56181 | | 22 | 0 | -1 | 1 | 0 | 15.5949 | 71.2910 | 0.21875 | | 23 | 1 | 0 | -1 | 0 | 13.5108 | 44.7365 | 0.30200 | | 24 | 0 | 0 | 0 | 0 | 12.4328 | 68.0930 | 0.18258 | | 25 | 0 | -1 | 0 | 1 | 11.5704 | 25.4405 | 0.4548 | | 26 | -1 | 1 | 0 | 0 | 14.1216 | 50.7014 | 0.2785 | | 27 | 0 | 0 | 0 | 0 | 11.3189 | 62.1640 | 0.1820 |
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