Antiprostate Cancer Activity of Ineupatolide Isolated from Carpesium cernuum L.

Figure 2

Effects of T-21 on the apoptosis and cell cycle of PC-3 cells. (a) PC-3 cells were treated with T-21 for 24 h, fixed, and stained with Hoechst. Nucleus changes and the formation of apoptotic bodies were observed using a fluorescence microscope (). (b, c) T-21 (10, 20, or 40 μM) was used to treat PC-3 cells for 24 h, which were then were fixed and stained with annexin V/PI. Flow cytometry was used to detect and analyze cell apoptosis in each group. The data are presented as (SDs; ). and vs. the control group. (d, e) PC-3 cells were treated with T-21 (10, 20, or 40 μM) for 24 h, fixed, and stained with PI. DNA content was detected and analyzed by flow cytometry. The percentage of PC-3 cells at different stages of the cell cycle is demonstrated by the bar graph. The data are presented as (). and vs. the control group.

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