BEZ235 Increases the Sensitivity of Hepatocellular Carcinoma to Sorafenib by Inhibiting PI3K/AKT/mTOR and Inducing Autophagy

Figure 2

Effects of sorafenib combined with BEZ235 on Huh7 or Huh7^R cell migration and colony formation. (a) Degree of scratch healing at 0, 24, and 48 h in each group. (SFB, 4 μM; BEZ235, 0.25 μM; and SFB, 4 μM + BEZ, 0.25 μM). ×100 magnification. (b) Huh7 or Huh7^R cells were incubated with various treatments for 24 h (SFB, 4 μM; BEZ235, 0.25 μM; and SFB, 4 μM + BEZ, 0.25 μM). The cell lysates were collected, and the designated proteins (MMP2, MMP9) were detected by western blot. (c) Colony formation assay was carried out to assess the proliferation of Huh7 and Huh7^R cells for 24 h (control; SFB, 1 μM; BEZ, 0.1 μM; and SFB 1 μM + BEZ 0.1 μM). Data are presented as , . , all versus SFB single agent. SFB: sorafenib; BEZ: BEZ235; SFB + BEZ: sorafenib combined with BEZ235.

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