Research Article

Fisetin Attenuates Lipopolysaccharide-Induced Inflammatory Responses in Macrophage

Figure 4

Effects of fisetin on MMP-2 and MMP-9 in macrophages stimulated with LPS. (a) mRNA expression of MMP-2 and MMP-9 in LPS- (10 ng/mL) stimulated macrophages in the presence or absence of fisetin (10-100 μM) by qPCR ( in each group). Each bar presents the of six experiments. vs. control group; # vs. LPS-stimulated group. (b) The activation of MMP-9 in LPS- (10 ng/mL) stimulated macrophages in the presence or absence of fisetin (10-100 μM) by gelatin zymography. The relative expression of MMP-9 activity was quantified by densitometry and normalized to the band under the control condition ( in each group). Each bar presents the of three experiments. .
(a)
(b)