Research Article
Senescence Osteoblast-Derived Exosome-Mediated miR-139-5p Regulates Endothelial Cell Functions
Figure 1
Establishment of primary osteoblast senescence model. (a) The condition of cells emerging from bone fragments under different days. (b) Observation of alkaline phosphatase staining in primary cells. (c) Analysis of β-galactosidase senescence staining in osteoblasts treated with D-gal for 48 h and quantitative analysis (). (d) Expression of p53 and p21 genes in osteoblasts. After D-gal induction ().
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