Research Article

Senescence Osteoblast-Derived Exosome-Mediated miR-139-5p Regulates Endothelial Cell Functions

Figure 3

Effect of osteoblast exosome-derived miR-139-5p on the biological function of vascular endothelial cells. (a) Expression of related miRNA in two types of cells treated by D-gal for 48 h. (b) Expression of miR-139-5p in vascular endothelial cells in the coculture system ( means comparison with the control group, ; # means comparison with the model, ). (c) Electron microscope scanning of exosome-isolated osteoblast cells and (d) particle size analysis of exosome western blot analysis (e) of exosome-enriched proteins (CD9 (22-25 kDa) and CD63 (26 kDa)) and the key proteins for miRNA function (TSG101 (45 kDa) and calnexin (90 kDa)). (f) Levels of miR-139-5p in exosomes isolated from the control and senescence groups of osteoblast cells were determined by qRT-PCR analysis (). (g) Senescence staining of vascular endothelial cells by the control and senescence groups of osteoblastic exosomes and quantitative analysis (). (h) Staining of the control and senescence groups of osteoblastic exosomes on apoptosis of vascular endothelial cells and quantitative analysis ().
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