Research Article
Factors Associated with Promoted Proliferation of Osteosarcoma by Peptidylarginine Deiminase 4
Figure 3
Increased expression of PADI4 in osteosarcoma tissues. (a) Expression levels of PADI4 protein in normal human osteoblast cell (hFOB1.19) and several human osteosarcoma cell lines (U2OS, Saos-2, HOS, SJSA1, and 143B) by Western blot. GAPDH was used as a loading control. (b) Western blot was performed to detect the expression levels of PADI4 in human osteosarcoma tissues and adjacent tissues. GAPDH was used as a loading control. (c) Quantification of protein expression. vs. adjacent tissues. (d) Expression of PADI4 mRNA in human osteosarcoma tissues and adjacent tissues was investigated by RT-PCR analysis. Data are presented as the . vs. adjacent tissues. (e) Immunohistochemistry staining of PADI4, p-ERK, and active β-catenin in human osteosarcoma tissues and adjacent tissues.
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