[Retracted] Cannabinoid WIN 55,212-2 Inhibits Human Glioma Cell Growth by Triggering ROS-Mediated Signal Pathways

Figure 3

WIN inhibits tumor spheroids growth of U251 cells ex vivo. (a) WIN dose-dependently inhibited tumor spheroids growth of U251 cells ex vivo. U251 cells were cultured in an ultralow attachment 6-well plate for 2 days. Then, cells were treated with 0-20 μM WIN for 48 h. (b) Win time-dependently caused ROS production in tumor spheroids of U251. Tumor spheroids of U251 were prelabeled by 10 μM DCFH-DA for 15 min, and then tumor spheroids of U251 were treated with 20 μM WIN for 0-120 min. ROS production was monitored by fluorescence microscope (magnification, × 100). Quantitative analysis of tumor spheroids volume (c) and ROS generation (d) were measured, respectively. All experiments were repeated three times. Bar with “” indicates the significant difference at level.

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