A Combination of Geniposide and Chlorogenic Acid Combination Ameliorates Nonalcoholic Steatohepatitis in Mice by Inhibiting Kupffer Cell Activation

Figure 6

GC inhibited the activation of RAW264.7 cell lines and decreased the expression of proinflammatory factors and chemokines. Toxicity test of GC RAW264.7 cell lines was performed by CCK8 measurement after cells were incubated with different treatment for 24 hours (a). NO content induced by different dosages of GC was test (b). TNF-α protein levels in cell were detected by immunoblotting (c). TNF-α, iNOS, IL-1β, and IL-6 mRNA expression in cells. Con: control diet; HFHC: high-fat/carbohydrate/cholesterol/choline diet; GC: geniposide and chlorogenic acid combination. , .

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