Overexpression of Long Noncoding RNA H19 Downregulates miR-140-5p and Activates PI3K/AKT Signaling Pathway to Promote Invasion, Migration and Epithelial-Mesenchymal Transition of Ovarian Cancer Cells

Figure 3

Validation of the relationship between H19 and miR-140-5p. (a) Predicted binding site. (b) DLR validated the targeting relationship between H19 and miR-140-5p. (c) RIP experiment. (d) MiR-140-5p expression level in A2780 and Anglne cells after transfection with H19, represents the comparison with the H19-sh group, ; ^# stands for the comparison with the H19-si group, . (e) Proliferation of A2780 cells after cotransfection of H19-si and miR-inhibitor. (f) Proliferation of Anglne cells after cotransfection of H19 and miR-140-5p. (g) Apoptosis rate and flow cytometry of A2780 and Anglne cells after cotransfection of H19 and miR-140-5p. (h) Invasion of A2780 and Anglne cells after cotransfection of H19 and miR-140-5p. (i) Migration of A2780 cells after cotransfection of H19 and miR-140-5p. (j) Migration of Anglne cells after cotransfection of H19 and miR-140-5p. represents compared with the H19-si group.

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