Downregulation of miR-497-5p Improves Sepsis-Induced Acute Lung Injury by Targeting IL2RB

Figure 3

Suppression of miR-497-5p reduced LPS-induced apoptosis and inflammatory cytokine production in BEAS-2B cells. LPS was used to stimulate BEAS-2B cells, and then cells were transfected with miR-497-5p inhibitor. (a) qRT-PCR for miR-497-5p level detection. (b) MTT assay for cell viability detection. (c) Flow cytometry for the ratio of apoptosis. (d) Western blot for apoptosis-related protein expression. qRT-PCR and ELISA for mRNA level (e) and content (f) of TNF-α, IL-1β, and IL-6 detection.

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