Research Article
Downregulation of miR-497-5p Improves Sepsis-Induced Acute Lung Injury by Targeting IL2RB
Table 1
The primer sequence used in qRT-PCR.
| | IL-1β (forward) | 5-CTCCGACCACCACTACAGCAAG-3 | | IL-1β (reverse) | 5-TGGGCAGGGAACCAGCATC-3 | | TNF-α (forward) | 5-CCCGAGTGACAAGCCTGTAGCC-3 | | TNF-α (reverse) | 5-CCCTTGAAGAGGACCTGGGAGTAGAT-3 | | IL-6 (forward) | 5-CAATGAGGAGACTTGCCTGGTG-3 | | IL-6 (reverse) | 5-GCTGGCATTTGTGGTTGGG-3 | | miR-497-5p (forward) | 5-CAGCAGCACTGTGGTTTGT-3 | | miR-497-5p (reverse) | 5-CGACAGCAGCACACTGTGGTT-3 | | SP1 (forward) | 5-TGGCAGCAGTACCAATGGC-3 | | SP1 (reverse) | 5-CCAGGTAGTCCTGTCAGAACTT-3 | | U6 (forward) | 5-CTCGCTTCGGCAGCACA-3 | | U6 (reverse) | 5-AACGCTTCACGAATTTGCTTC-3 | | GAPDH (forward) | 5-TCAAGGCTGAGAACGGGAAG-3 | | GAPDH (reverse) | 5-TGGACTCCACGACGTACTCA-3-3 |
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