Research Article

Quercetin Alleviates Intervertebral Disc Degeneration by Modulating p38 MAPK-Mediated Autophagy

Figure 2

QUE protects NPCs against TBHP-induced apoptosis and ECM degradation. (a) Measurement of intracellular ROS levels by flow cytometry. (b) Quantification of ROS levels by measuring the DCFH-DA fluorescence intensity in each treatment group. (c) Western blot analysis of the prosurvival protein, Bcl-2, and proapoptotic protein, Bax, and ECM-related proteins, collagen II, ACAN, and MMP13. (d) Quantification of protein levels for Bcl 2, Bax, collagen II, ACAN, and MMP13. Data are presented as the from three independent experiments. ; ; .
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