BioMed Research International

BioMed Research International / 2021 / Article

Research Article | Open Access

Volume 2021 |Article ID 6646664 |

Jiaqiang Niu, Keiwei Li, Huachun Pan, Xing Gao, Jiakui Li, Dongjing Wang, Mingshuai Yan, Yefen Xu, Suolang Sizhu, "Epidemiological Survey of Mycoplasma bovis in Yaks on the Qinghai Tibetan Plateau, China", BioMed Research International, vol. 2021, Article ID 6646664, 4 pages, 2021.

Epidemiological Survey of Mycoplasma bovis in Yaks on the Qinghai Tibetan Plateau, China

Academic Editor: Maria Bayliak
Received18 Dec 2020
Revised24 Feb 2021
Accepted15 Mar 2021
Published14 Apr 2021


Mycoplasma bovis (M. bovis) is one of the most important pneumonia pathogens in yaks. It may result in more economic losses due to the cold and anoxia condition at Qinghai Tibetan plateau. However, to date, limited information on M. bovis infection in yaks is available in China. For this purpose, the seroprevalence of M. bovis was investigated in yaks living in the mentioned area through commercial ELISA kits. A total of 959 yaks were incorporated into this study. The prevalence of the disease in yaks was 48.70%. The serological results revealed a relatively high prevalence of M. bovis infection in yaks. The present study may greatly contribute to the prevention of this disease. More importance should be given to the potential threat caused by M. bovis in the special plateau.

1. Introduction

Qinghai Tibetan plateau is a particular environment with high altitude, cold, and anoxic conditions [1]. Yak (Bos grunniens) is a unique bovine species that is mainly distributed in this plateau. More than 18 million yaks live on the Qinghai-Tibet plateau, whether only a few yaks are found in India, Bhutan, Sikkim, Afghanistan, and Pakistan [2]. This unique species is essential for Tibetans because of the associated milk, wool, and meat production [3].

In 1961, Mycoplasma bovis (M. bovis) was isolated in America and first described as a cause of respiratory disease in 1976 [4]. Due to M. bovis, infection resulted in high economic losses every year due to pneumonia, mastitis, arthritis, keratoconjunctivitis, otitis, reproductive tract inflammation, abortion, and infertility [5]. In 2008, M. bovis was first isolated from the lungs of taurine cattle (Bos taurus) with pneumonia in China. Since then, it had broken out in some areas of China [6]. The incidence and mortality rates were 50-100% and 10-50%, respectively [7]. With the yak industry’s development, large-scale farming is expanding rapidly, which leads to the prevalence of significant infectious diseases on the Qinghai-Tibet plateau. However, to date, limited information on M. bovis infection in yaks is available on the Qinghai-Tibet plateau [8].

In this study, enzyme-linked immunosorbent assay (ELISA) kits were used to investigate the prevalence of M. bovis in yaks on the Qinghai-Tibet plateau. The information about the prevalence of M. bovis on this plateau may be of interest to the livestock owners and relevant departments. It may provide a certain reference for this disease’s prevention and control strategies.

2. Materials and Methods

2.1. Ethics Statement

All procedures were approved and performed by the Laboratory Animals Research Centre of Hubei, Qinghai, Sichuan, Gansu, and Tibet in China and the Ethics Committee of Tibet Agriculture and Animal Husbandry College, China. All animal experiments and procedures were conducted under the relevant guidelines of Proclamation of the Standing Committee of Tibet People’s Congress, China.

2.2. Serum Samples

Blood samples from 959 yaks were collected on the Qinghai-Tibet plateau during June 2019 to September 2020 (Qinghai: 224, Gansu: 243, Tibet: 436, and Sichuan: 56) (Figure 1). All the animals that were investigated in this study had no obvious clinical symptoms. Samples were collected mainly from the highland grazing areas, where owners have not recorded obvious symptoms in their herds. Also, herders did not take any protective measures to prevent and cure diseases. The detail information related to the region, gender, and age was reported on prescribed proforma. All the blood samples were centrifuged at 1000 rpm for 10 min, and then, serum was separated and stored at −20°C until further analysis.

2.3. Determination of Antibodies against M. bovis

All serum samples were tested for anti-M. bovis according to the manufacturer’s instructions, bovis antibodies by using two commercial ELISA kits (one was from IDEXX Laboratories, Inc, Beijing, China; the other one came from Shanghai Enzyme-linked Biotechnology Co., Ltd., Shanghai, China). The test value was based on the optical density values at 450 nm (OD 450). To ensure validity, the average OD 450 of positive controls was ≥1.00; the average OD 450 of negative controls was ≤0.15. The positive and negative controls were endowed in the kit and included in each test. The cutoff value was equal to the average OD 450 of negative controls plus 0.15. The results were considered positive when the OD 450 was higher or equal to the cutoff value and considered negative when the OD 450 was lower than the cutoff value. It was judged to be positive when both results were positive and vice versa. In the case of only one positive out of two, IDEXX was used for reexamination.

2.4. Statistical Analysis

A multivariable logistic regression model (MLRM) was used to assess significant variables (region, gender, and age) by using the IBM SPSS Statistics 25.0 (SPSS Somers, NY). Statistically significant levels within factors and interactions were accepted when the probability value was found ≤0.05. Odds ratios (OR) was kept at 95% confidence intervals (CI).

3. Results

A total of 467/959 (48.70%) yaks were reported positive for M. bovis. In Qinghai, Gansu, Tibet, and Sichuan, the prevalence was 45.09%, 53.09%, 46.10%, and 64.29%, respectively. Regarding gender, the prevalence in male and female was 45.71% and 50.24%, respectively. If we consider the yaks’ age, the prevalence ranged from 45.23% to 55.70% (Table 1).

VariableCategoryNumber of samplesPositive samples% (95% CI) valueOR (95% CI)

RegionQinghai22410145.09 (38.45-51.86)Reference
Gansu24312953.09 (46.60-59.50)0.081.38 (0.96-1.98)
Tibet43620146.10 (41.35-50.91)0.811.04 (0.75-1.44)
Sichuan563664.29 (50.36-76.64)0.012.19 (1.20-4.02)

GenderMale32614945.71 (40.21-51.28)Reference
Female63331850.24 (46.27-54.20)0.181.20 (0.92-1.57)

Age1588855.70 (47.59-63.58)0.041.52 (1.03-2.25)
1848546.20 (38.83-53.68)0.841.04 (0.72-1.51)
28312845.23 (39.33-51.23)Reference
33416649.70 (44.21-55.19)0.271.20 (0.87-1.64)

Total95946748.70 (45.49-51.91)

According to conditional stepwise logistic regression, region, gender, and age were assessed as potential risk factors in the present study. Furthermore, Sichuan’s yaks had a 2.19 times higher risk of infection than yaks in Qinghai () (Table 1). Yaks in Gansu showed 1.38 times higher risk of infection than yaks in Qinghai (Table 1). Meanwhile, yaks having <1 year age had a 1.52 times higher risk of infection as compared to yaks in () (Table 1 and Figure 2), while no significant difference was found in male and female yaks (Table 1 and Figure 3).

4. Discussion

The bovine industry has experienced many outbreaks caused by M. bovis worldwide [9]. Previous reports showed that more than 30% of pneumonia was caused by M. bovis in Europe, and the incidence rate was high (70%) in America [10]. At present, M. bovis has distributed worldwide; it also had reported in many regions of China. In our study, the overall prevalence of M. bovis in yaks was 48.70%. Similar levels of M. bovis infection were documented in Shanghai (42.1%) [11]. M. bovis infection prevalence was less than taurine cattle in the Xinjiang Uyghur Autonomous Region (86.3%) [12].

In different regions, the prevalence of M. bovis in Sichuan was higher than in Tibet, Gansu, and Qinghai. The highest prevalence was detected in calf because of the weak resistance, strong stress response, and vertical transmission [13]. In general, the prevalence of M. bovis was no significant difference in gender. But in Gansu and Sichuan, the prevalence of M. bovis in females was higher than that in males which might be due to the weak resistance, especially during pregnancy and parturition. These findings agree with the report by Xie et al. [14].

With the yak industry’s rapid development, the high infection of M. bovis in yaks might be due to the frequent trade and lack of strict quarantine. In addition, drug resistance is an increasing problem worldwide, and there are few commercial vaccines for the prevention of M. bovis [15].

In conclusion, this study described a high prevalence rate of M. bovis infection in yaks on the Qinghai-Tibet plateau. Therefore, more attention should be paid to assess the impact of M. bovis infection fully. Effective measures should also be taken to control the spread of M. bovis by considering the role of various factors [16].

Data Availability

All the data in the current study could be available by contacting the corresponding author.

Conflicts of Interest

The authors state that there are no competing interests.


This study was supported by the Chinese Agricultural Research Service (CARS-37) and the key research and development program of Tibet Autonomous Region (XZ202001ZY0046N).


  1. X. Gao, L. Zhang, X. Jiang et al., “Porcine epidemic diarrhea: an emerging disease in Tibetan pigs in Tibet, China,” Tropical Animal Health and Production, vol. 51, no. 2, pp. 491–494, 2019. View at: Publisher Site | Google Scholar
  2. X. Gao, L. Zhang, X. Tong et al., “Epidemiological survey of fasciolosis in yaks and sheep living on the Qinghai- Tibet plateau, China,” Acta Tropica, vol. 201, p. 105212, 2020. View at: Publisher Site | Google Scholar
  3. M. Wang, Y. H. Wang, Q. Ye, P. Meng, H. Yin, and D. L. Zhang, “Serological survey of Toxoplasma gondii in Tibetan mastiffs (Canis lupus familiaris) and yaks (Bos grunniens) in Qinghai, China,” Parasite & Vectors, vol. 5, no. 1, p. 35, 2012. View at: Publisher Site | Google Scholar
  4. M. B. Petersen, J. Pedersen, D. L. Holm, M. Denwood, and L. R. Nielsen, “A longitudinal observational study of the dynamics of Mycoplasma bovis antibodies in naturally exposed and diseased dairy cows,” Journal of Dairy Science, vol. 101, no. 8, pp. 7383–7396, 2018. View at: Publisher Site | Google Scholar
  5. D. Vojinovic, N. Zdravkovic, R. Prodanović et al., “Seroprevalence of Mycoplasma bovis in grazing dairy cows from five different areas in Serbia,” Journal of the Hellenic Veterinary Medical Society, vol. 69, no. 4, p. 1241, 2019. View at: Publisher Site | Google Scholar
  6. J. Q. Xin, Y. Li, D. Guo et al., “First isolation of Mycoplasma bovis from calf lung with pneumoniae in China,” Chinese Journal of Preventive Veterinary Medicine, vol. 30, no. 9, 2008. View at: Google Scholar
  7. K. LI, Z. Q. Han, J. F. Gao, M. Y. Liu, D. Zhang, and J. K. Li, “Seroprevalence of Mycoplasma infection in cattle in some counties of Tibet, China,” China Dairy Cattle, vol. 16, pp. 8–10, 2014. View at: Google Scholar
  8. T. Wei, “Serological investigation of Mycoplasma yak pneumonia in Yushu County, Qinghai Province,” China Animal Health, vol. 14, no. 8, p. 47, 2012. View at: Google Scholar
  9. H. Wang and H. Wu, “Progress in the study of bovine Mycoplasma,” Chinese Dairy Cattle, vol. 7, pp. 36–41, 2014. View at: Google Scholar
  10. Q. Shao, Epidemiological Investigation and Research on Prevention and Control Technology of Bovine Mycoplasma Disease in Yongning County, Ningxia, Gansu Agricultural University, 2017.
  11. Q. Shen, H. Qu, C. Yu et al., “Serological investigation of bovine Mycoplasma infection in dairy cows,” Shanghai Journal of Animal Husbandry and Veterinary Medicine, vol. 6, pp. 33-34, 2011. View at: Google Scholar
  12. J. Li, Y. Li, W. Fan et al., “Serological surveys of 5 infectious diseases in several large-scale dairy cow farms in Xinjiang,” Progress in Veterinary Medicine, vol. 34, no. 11, pp. 24–27, 2013. View at: Google Scholar
  13. T. Prysliak, T. Maina, and J. Perez-Casal, “Th-17 cell mediated immune responses to Mycoplasma bovis proteins formulated with Montanide ISA61 VG and curdlan are not sufficient for protection against an experimental challenge with Mycoplasma bovis,” Veterinary Immunology & Immunopathology, vol. 197, pp. 7–14, 2018. View at: Publisher Site | Google Scholar
  14. J. Xie, Q. Hou, H. Jiang et al., “Seroepidemiological investigation of Mycoplasma bovis in Chongqing from 2008 to 2013,” Heilongjiang Animal Husbandry and Veterinary Science, vol. 12, pp. 87–89, 2015. View at: Google Scholar
  15. J. Perez-Casal, T. Prysliak, T. Maina, M. Suleman, and S. Jimbo, “Status of the development of a vaccine against Mycoplasma bovis,” Vaccine, vol. 35, no. 22, pp. 2902–2907, 2017. View at: Publisher Site | Google Scholar
  16. G. Wiener, “The yak, an essential element of the high altitude regions of Central Asia,” Etudes Mongoles et Siberiennes, vol. 43, pp. 43-44, 2013. View at: Google Scholar

Copyright © 2021 Jiaqiang Niu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Related articles

No related content is available yet for this article.
 PDF Download Citation Citation
 Download other formatsMore
 Order printed copiesOrder

Related articles

No related content is available yet for this article.

Article of the Year Award: Outstanding research contributions of 2021, as selected by our Chief Editors. Read the winning articles.