Assessment of Developmental and Reproductive Fitness of Dengue-Resistant Transgenic Aedes aegypti and Improvement of Fitness Using Antibiotics
Developmental fitness parameter measurements of transgenic mosquito (TM) in comparison to wild mosquitoes (WM): (a) oviposition, (b) fertility, (c) larval life span, (d) adult male life span, and (e) adult female life span. Survival curves were compared between WM and TM. Approximately, 200 mosquitoes of TM and WM were used in this experiment. Oviposition defined as the number of eggs per blood-fed female mosquitoes and female mosquitoes without any eggs laid was excluded from results; fecundity as the number of L1 larvae/number of eggs; larval life span as the number of days to become pupae from larvae; and adult life span as the number of days to the death of the mosquitoes. Three replicates were performed for each experiment. The Mann–Whitney test was performed to analyze the parameters between WM and TM. Bars represent maximum value to the minimum value of the results of TM or WM. Significant differences at are indicated by .