F1012-2 Induced ROS-Mediated DNA Damage Response through Activation of MAPK Pathway in Triple-Negative Breast Cancer

<div>F1012-2 induces DNA damage in MDA-MB-231 and MDA-MB-468 cells. (a) The cells were treated with F1012-2 (0, 4, and 8 <i>μ</i>g/ml) for 24 hours, and then, DNA damage (strand breaks) was determined with comet assay. (b) After 24 hours of treatment with F1012-2, total cellular protein was extracted from two cells, and the levels of <i>γ</i>-H2AX were detected by western blotting. (c) The representative images of <i>γ</i>-H2AX immunofluorescence staining in two cells treated with F1012-2 for 24 hours. <span class="nowrap"><svg height="12.7373pt" id="M16" style="vertical-align:-3.429399pt" version="1.1" viewbox="-0.0498162 -9.3079 98.2531 12.7373" width="98.2531pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M409 504C401 567 396 607 392 642C354 654 312 665 266 665C137 665 60 583 60 487C60 374 161 325 225 290C300 250 355 215 355 141C355 68 311 21 235 21C131 21 86 122 71 183L41 176C48 128 61 42 68 21C78 16 93 8 118 0C142 -7 175 -15 216 -15C349 -15 438 69 438 174C438 287 344 333 265 374C186 414 138 449 138 522C138 576 172 631 249 631C336 631 363 562 380 499L409 504Z"></path></g><g transform="matrix(.013,0,0,-0.013,6.345,0)"><path d="M390 111C344 68 312 56 269 56C212 56 118 102 118 241C118 346 175 401 241 401C277 401 312 388 342 360C350 352 355 349 361 349C372 349 394 371 394 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BioMed Research International

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Figure 2

Figure 2: F1012-2 Induced ROS-Mediated DNA Damage Response through Activation of MAPK Pathway in Triple-Negative Breast Cancer 