Research Article

F1012-2 Induced ROS-Mediated DNA Damage Response through Activation of MAPK Pathway in Triple-Negative Breast Cancer

Figure 4

F1012-2 activates the MAPK pathway in MDA-MB-231 and MDA-MB-468 cells. (a) Total cell proteins were extracted after treatment with F1012-2, and protein levels were detected by western blot. (b) The cells were pretreated with SP600125, PD98059, and SB203580 for 30 minutes and then incubated with F1012-2. Western blotting was used to detect protein expression. (c) The cells were pretreated with inhibitors; then, the cell viability was measured by the MTT assay. and .
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