lncRNA LINC01296 Promotes Oral Squamous Cell Carcinoma Development by Binding with SRSF1

Figure 2

LINC01296 promotes the proliferation, migration, invasion, and EMT process of OSCC cells in vitro. (a) qRT-PCR was used to detect the relative level of LINC01296 in CAL-27 and HN30 cells 24 hours after transfection of SS-LINC01296. . (b) qRT-PCR was used to detect the relative stable overexpression of LINC01296 in CAL-27 and SCC-9 cells. . (c) The MTT method was used to detect the proliferation activity of CAL-27 and HN30 cells transfected with SS-LINC01296 and (d) CAL-27 and SCC-9 cells with LINC01296 overexpressing. . The colonization ability of cells transfected with SS-LINC01296 (e) and cells with LINC01296 overexpressing (f). . The transwell method was used to detect the migration and invasion ability of transfected with SS-LINC01296 in CAL-27 and HN30 cells (g) and CAL-27 and SCC-9 cells (h) stably overexpressing LINC0129. . Western blot was used to detect the E-cadherin, N-cadherin, and vimentin proteins after downregulation of LINC01296 (i) or overexpression of LINC01296 (j) in OSCC cells. . QRT-PCR was used to detect the E-cadherin, N-cadherin, and vimentin mRNA after downregulation of LINC01296 (k) or overexpression of LINC01296 (l) in OSCC cells. . NC: negative control; SS-LINC01296: LINC01296 smart silencer. ; .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)