lncRNA LINC01296 Promotes Oral Squamous Cell Carcinoma Development by Binding with SRSF1

Figure 4

Physical interaction between SRSF1 and LINC01296. (a) The localization of LINC01296 in CAL-27 cells was analyzed by FISH. The nucleus was stained with DAPI, and the cytoplasm was labeled with 18s. (b) Binding sites and motifs were predicted. (c) Expression of SRSF1 in head and neck cancer was predicted by GEPIA. (d) Correlation between LINC01296 and SRSF1 expression in head and neck cancer. (e) The PCR method was used to detect the effect of LINC01296 on SRSF1 mRNA. . (f) The effect of LINC01296 overexpression on the SRSF1 protein level was measured by western blot. . (g) The effect of silencing LINC01296 on the level of SRSF1 protein was detected by western blot. . (h) The binding relationship between LINC01296 and SRSF1 protein was verified by RNA pull-down assay. (i) The RIP experiment further proved the binding relationship between LINC01296 and SRSF1. ; NS: no significant.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)