Selection and Evaluation of Potential Reference Genes for Quantitative Real-Time PCR in Agaricus blazei Based on Transcriptome Sequencing Data

Table 4

Expression stability of candidate reference genes obtained from BestKeeper analysis.

Rank	5 stages		Fruit body		Stipe		Pileus		Total
	Gene	SD [±CP]	Gene	SD [± CP]	Gene	SD [± CP]	Gene	SD [± CP]	Gene	SD [± CP]
1	ATP3	0.962	Copb1	0.627	FKS1	0.769	ATP3	0.368	ATP7	0.837
2	Vps26	0.788	Cdc4	0.564	NdufS7	0.647	Cdc4	0.418	ATP3	0.882
3	Uqcr8	0.978	ATP3	0.613	ATP7	0.656	α-TUB	0.409	Uqcrc	0.963
4	Cdc4	0.859	NdufS7	0.521	ATP3	0.631	Uqcrc	0.158	Copb1	0.906
5	α-TUB	0.981	ATP7	0.552	Uqcrc	0.668	Cox5a	0.401	Vps26	0.676
6	CS	0.962	α-TUB	0.534	Cdc4	0.693	SPRYp	0.527	Cdc4	0.843
7	NdufS7	0.811	NDUFB9	0.660	α-TUB	0.571	CS	0.370	Cox5a	0.828
8	ATP2	0.911	Vps26	0.387	Ras	0.731	CPR1	0.301	NdufS7	0.760
9	FKS1	0.909	Uqcr8	0.567	ATPDH	0.673	β-TUB2	0.527	FKS1	0.819
10	Gnb2	0.867	Uqcrc	0.574	CPR1	0.488	Ras	0.376	CS	0.840
11	PUP3	0.818	Cox5a	0.477	PUP3	0.520	Vps26	0.207	ATP2	0.703
12	RPT6	0.799	CPR1	0.643	Copb1	0.612	ATPDH	0.411	PUP3	0.785
13	NDUFB9	0.793	PUP3	0.592	CS	0.649	ATP2	0.249	ATP1	0.856
14	ATPDH	0.966	RPT6	0.643	ATP1	0.647	NdufS7	0.231	Ras	0.863
15	CPR1	0.804	CS	0.542	Uqcr8	0.589	Uqcr8	0.180	Uqcr8	0.733
16	PsmB6	0.894	ATP2	0.462	Gnb2	0.711	NDUFB9	0.244	α-TUB	0.777
17	SPRYp	0.763	ATP1	0.504	PsmB6	0.518	NdufA2	0.212	ATPDH	0.822
18	β-TUB2	0.766	Gnb2	0.536	ATP2	0.470	FKS1	0.277	CPR1	0.670
19	NdufA2	0.380	SPRYp	0.636	Cox5a	0.707	ATP1	0.340	Gnb2	0.823
20	ATP7	1.009	FKS1	0.463	SPRYp	0.595	Copb1	0.176	NDUFB9	0.800
21	Copb1	1.256	PsmB6	0.647	Vps26	0.554	RPT6	0.261	RPT6	0.737
22	Uqcrc	1.152	Ras	0.554	RPT6	0.593	Gnb2	0.319	PsmB6	0.709
23	Cox5a	1.008	ATPDH	0.531	NDUFB9	0.701	PsmB6	0.347	SPRYp	0.697
24	ATP1	1.043	β-TUB2	0.563	GAPDH	0.660	PUP3	0.129	NdufA2	0.451
25	Ras	1.035	NdufA2	0.225	β-TUB2	0.996	ATP7	0.160	β-TUB2	1.059
26	GAPDH	1.149	GAPDH	1.089	NdufA2	0.445	GAPDH	1.094	GAPDH	1.026