Quantitative Phosphoproteomic Comparison of Lens Proteins in Highly Myopic Cataract and Age-Related Cataract

Table 4

Phosphosites differentially expressed in HMC-N vs ARC-N.

Protein names	Gene name	Accession	Score	Modified sequence	Position	FC	value
Phosphosites exclusively detected in HMC-N
Retinal dehydrogenase 1	ALDH1A1	P00352	78.92	_YILGNPLT(ph)PGVTQGPQIDKEQYDK_	337	NaN	0.019
Fructose-bisphosphate aldolase	ALDOA	J3KPS3	92.46	_RTVPPAVTGITFLS(ph)GGQSEEEASINLNAINK_	276	NaN	0.008
Fructose-bisphosphate aldolase	ALDOA	J3KPS3	80.23	_CQY(ph)VTEK_	208	NaN	≤0.001
Cysteine protease	ATG4D	B4DZK0	64.3	_KYS(ph)IFTEKDEILSDVASR_	151	NaN	0.014
Carbonic anhydrase	CA2	P00918	61.44	_EPIS(ph)VSSEQVLK_	216	NaN	0.001
Alpha-crystallin A chain	CRYAA	P02489	142.5	_HFSPEDLT(ph)VK_	86	NaN	≤0.001
Beta-crystallin A4	CRYBA4	P53673	86.48	_GFQYVLECDHHS(ph)GDYK_	170	NaN	≤0.001
Beta-crystallin A4	CRYBA4	P53673	60.56	_GEY(ph)PSWDAWGGNTAYPAER_	74	NaN	≤0.001
Beta-crystallin B3	CRYBB3	P26998	69.12	_CELS(ph)AECPSLTDSLLEK_	42	NaN	≤0.001
Quinone oxidoreductase	CRYZ	Q08257	98.77	_AGESVLVHGAS(ph)GGVGLAACQIAR_	158	NaN	0.011
Eukaryotic initiation factor 4A-II	EIF4A2	Q14240	137.9	_GYDVIAQAQS(ph)GTGK_	79	NaN	0.001
S-Formylglutathione hydrolase	ESD	P10768	117.4	_MSIFGHS(ph)MGGHGALICALK_	149	NaN	≤0.001
Glyceraldehyde-3-phosphate dehydrogenase	GAPDH	P04406	68	_IISNASCT(ph)TNCLAPLAK_	153	NaN	0.012
Glutathione synthetase	GSS	P48637	156.7	_QIEINTIS(ph)ASFGGLASR_	149	NaN	0.003
Inosine-5-monophosphate dehydrogenase	IMPDH1	Q5H9Q6	121.6	_LVGIVT(ph)SR_	234	NaN	0.009
Phakinin	BFSP2	Q13515	116.4	_S(ph)SSS(ph)LESPPASR_	35	NaN	0.015
Protein kinase C and casein kinase substrate in neurons 3	PACSIN3	D3DQR0	92.54	_LKEVEAS(ph)K_	153	NaN	0.032
Peroxisome biogenesis factor 10, isoform CRA_b	PEX10	A0A024R0A4	87.26	_RAS(ph)LEER_	281	NaN	≤0.001
HSPC141	PHPT1	Q9P019	66.87	_(ac)AVADLALIPDVDIDS(ph)DGVFK_	16	NaN	0.001
Plectin	PLEC	Q15149	93.16	_LS(ph)FSGLR_	3441	NaN	0.019
Plectin	PLEC	Q15149	69.72	_KAS(ph)DSELER_	2039	NaN	≤0.001
Glycogen phosphorylase, liver form	PYGB	P06737	61.64	_RMS(ph)LIEEEGSKR_	430	NaN	0.036
SEC14-like protein 2	SEC14L2	O76054	122.5	_VGDLS(ph)PR_	9	NaN	≤0.001
Ubiquitin C variant (fragment)	UBC	Q59EM9	122.4	_TIT(ph)LEVEPSDTIENVK_	30	NaN	0.009
14-3-3 protein zeta/delta (fragment)	YWHAB	E7EX29	55.89	_DICNDVLS(ph)LLEK_	99	NaN	≤0.001
Phosphosites exclusively detected in ARC-N
Alpha-crystallin B chain	CRYAA	P02511	127	_LFDQFFGEHLLESDLFPTSTSLS(ph)PFYLRPPSFLR_	2	NaN	0.010
Beta-crystallin A3	CRYBA1	P05813	103.2	_WDAWSGSNAY(ph)HIER_	174	NaN	0.009
3-Hydroxyanthranilate 3,4-dioxygenase	HAAO	P46952	78.43	_RLS(ph)LAPDDSLLVLAGTSYAWER_	160	NaN	0.007
Protein NDRG1	NDRG1	Q92597	96.54	_S(ph)REMQDVDLAEVKPLVEK_	56	NaN	≤0.001
Phosphosites with upregulation in HMC-N (selected)
Alpha-crystallin B chain	CRYAA	P02511	177.9	_RPFFPFHSPS(ph)R_	21	####	0.020
Alpha-crystallin B chain	CRYAA	P02511	84.3	_EEKPAVT(ph)AAPK_	170	####	0.000
Alpha-crystallin B chain	CRYAA	P02511	151.2	_LEKDRFS(ph)VNLDVK_	76	####	0.004
Beta-crystallin B1	CRYBB1	P53674	154.7	_QWHLEGSFPVLAT(ph)EPPK_	248	####	≤0.001
Filensin	BFSP1	Q12934	58.89	_KEQYEHADEAS(ph)R_	22	8.20	0.002
Phosphosites with downregulation in HMC-N
Phosphoglycerate kinase 1	PGK1	P00558	69.38	_AHS(ph)S(ph)MVGVNLPQK_	174	0.38	0.005
Carbonyl reductase (NADPH) 1	CBR1	P16152	228.2	_FRS(ph)ETITEEELVGLMNK_	160	0.47	0.008
Carbonyl reductase (NADPH) 1	CBR1	P16152	207.6	_GQAAVQQLQAEGLS(ph)PR_	56	0.47	0.019
Beta-crystallin S	CRYGS	P22914	190.8	_KPIDWGAASPAVQS(ph)FRR_	172	0.49	0.016