Research Article

lncRNA MALAT1 Regulates Mouse Granulosa Cell Apoptosis and 17β-Estradiol Synthesis via Regulating miR-205/CREB1 Axis

Figure 3

MALAT1 is targeted by miR-205 in mGCs. (a) The predicted miR-205 binding sites in the region of MALAT1 and the corresponding mutant sequences are shown. (b) Effect of miR-205 on the luciferase activity of WT-MALAT1 and MT-MALAT1 reporter systems was detected via luciferase reporter assay. (c) RNA pull-down assay was conducted to assess the relationship between miR-205 and MALAT1. (d) The expression of miR-205 was examined in mGCs transfected with si-MALAT1 or si-NC. (e) The expression of miR-205 was examined in mGCs transfected with miR-205 mimics, miR-NC, miR-205 inhibitor (anti-miR-205), and anti-miR-NC. (f) The expression of MALAT1 was examined in mGCs transfected with miR-205 mimics, miR-NC, miR-205 inhibitor (anti-miR-205), and anti-miR-NC. Data are the () of one representative experiment, , vs. the si-NC group. mGCs: mouse granulosa cells; MALAT1: metastasis-associated lung adenocarcinoma transcript 1.
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