The Important Role of Metal Ions for Survival of Francisella in Water within Amoeba Environment

Figure 1

Influence of different concentrations of manganese and zinc on the growth kinetics of F. novicida in water samples 1 (a) and 2 (b). Zinc and manganese ions were added in ultrapure water at different concentrations (0.1 μM, 0.8 μM, 0.1 mM, 0.5 mM, and 1.0 mM), followed by inoculation of the bacteria at concentration of 10⁷ CFU/ml. The experiments were performed at room temperature. At days 1, 2, 3, 4, and 5, after the inoculation of the bacteria, the CFU/milliliter of F. novicida was determined by plating the serial dilutions on BCYE agar at 37°C. The growth kinetics of bacteria in ultrapure water without metal ions was considered as control. Experiments were performed in triplicate and repeated three times. Student -test, was accepted as significantly different from the control sample.

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