The Important Role of Metal Ions for Survival of Francisella in Water within Amoeba Environment

Figure 3

The growth kinetics of F. novicida in water samples 1 (a) and 2 (b) as well as A. castellanii in water samples 1 (c) and 2 (d) during a coincubation. F. novicida and A. castellanii were coincubated in water samples at room temperature for 30 days. Growth kinetics of bacteria and amoeba were followed every five days in a period of 30 days. Water samples with addition of F. novicida without amoebae and A. castellanii without bacteria were used as a control. The number of F. novicida at each time point was determined by plating serial dilutions on BCYE agar plates. Amoeba cells were counted using light microscopy. Experiments were performed in triplicate and repeated three times. Student -test, was accepted as significantly different from the control sample.

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