The Important Role of Metal Ions for Survival of Francisella in Water within Amoeba Environment

Figure 5

Determination of the concentration of manganese and zinc in water samples 1 (a, b) as well as in water sample 2 (c, d) after a coincubation. Before inoculation of the bacteria and/or amoeba, the initial concentrations of transition metals Zn and Mn were determined in all water samples using an atomic spectrometer (AAS, Perkin Elmer Analyst 200, SAD 2003). Analyses were performed according to the standards accepted by the Croatian Standards Institute. Water samples without addition of metal ions were used as a control. All experiments were performed in triplicate and repeated three times. Student -test, was accepted as significantly different from control samples without addition of bacteria.

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