Comparison of Cell-Free Extracts from Three Newly Identified Lactobacillus plantarum Strains on the Inhibitory Effect of Adipogenic Differentiation and Insulin Resistance in 3T3-L1 Adipocytes

Figure 6

Effects of LS, LP, and L14 extracts on the amount of glucose uptake into 3T3-L1 adipocytes and insulin resistance-related protein expressions. (a) 2-NBDG, a fluorescent-tagged glucose analog, was used to evaluate the efficacy of transport to adipocytes. The treatment of 1 μM insulin was used as a positive control. (b) The amount of the glucose uptake by three extracts at a concentration of 100 μg/mL was detected using the fluorescence microplate reader (). (c) Western blot for insulin resistance-related proteins (TNF-α, AKT, and p-AKT) levels (). (d) Quantitative analysis of p-AKT and TNF-α expression levels in (c). GAPDH was used for normalization. Error bars represent the standard deviation of the mean. , , , one-way ANOVA followed by Tukey’s post hoc test was used. . PC: positive control.

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