Research Article
miR-125a-5p Regulates Osteogenic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells under Oxidative Stress
Table 1
Primer sequences for qRT-PCR.
| | Gene or miRNA | Sequence(5 to 3) |
| | miR-125a-5p | F: CGATTCCCTGAGACCCTTTAA | | ALP | F: CGAGATACAAGCACTCCCACTTC | | R: CTGTTCAGCTCGTACTGCATGTC | | Runx2 | F: CAAGGACAGAGTCAGATTAC | | R: GTGGTAGAGTGGATGGAC | | OCN | F: GGTGCAGCCTTTGTGTCCAAGC | | R: GTCAGCCAACTCGTCACAGTCC | | Osterix | F: TGCTTGAGGAGGAAGTTC | | R: CTTTGCCCAGAGTTGTTG | | p16 | F: GATCCAGGTGGGTAGAAGGTC | | R: CCCCTGCAAACTTCGTCCT | | p21 | F: TGTCCGTCAGAACCCATGC | | R: AAAGTCGAAGTTCCATCGCTC | | p53 | F: CAGCACATGACGGAGGTTGT | | R: TCATCCAAATACTCCACACGC | | GAPDH | F: CCATCTTCCAGGAGCGAGATC | | R: GCCTTCTCCATGGTGGTGAA | | U6 | F: CTCGCTTCGGCAGCACA | | R:AACGCTTCACGAATTTGCGT |
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