Circular RNA circPVT1 Contributes to Doxorubicin (DXR) Resistance of Osteosarcoma Cells by Regulating TRIAP1 via miR-137

Figure 5

TRIAP1 served as a target of miR-137. (a, b) Relative TRIAP1 mRNA expression was examined in 52 osteosarcoma tissues, 45 normal tissues, chemosensitive group (31), and chemoresistant group (21). (c, d) TRIAP1 protein levels were assessed in 52 osteosarcoma tissues, 45 normal tissues, chemosensitive group (31), and chemoresistant group (21). (e, f) Both mRNA level and protein level of TRIAP1 were measured in hFOB1.19, KHOS, KHOS/DXR, U2OS, and U2OS/DXR cells. (g) Pearson correlation analysis was applied to assess the expression association between TRIAP1 and miR-137 in osteosarcoma tissues. (h) The binding sites between miR-137 and TRIAP1 3UTR wt and the sequences of TRIAP1 3UTR mut. (i) Dual-luciferase reporter assay was used to prove the potential binding relationship between miR-137 and TRIAP1 in 293T cells. (j) The interaction between miR-137 and TRIAP1 3UTR in KHOS/DXR and U2OS/DXR cells was confirmed by using the RIP assay. (k) miR-137 level was detected in KHOS/DXR and U2OS/DXR cells transfected with mimic NC and miR-137 mimic. .

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