Inhibition of the mTORC1/NF-κB Axis Alters Amino Acid Metabolism in Human Hepatocytes

Figure 1

Rapamycin inhibits mTORC1 signaling and NF-κB activation and attenuates the expression of AST, GDH, GAD, and ODC in hepatocytes. (a) The phosphorylation of mTOR and S6 was decreased by rapamycin treatment. (b) The phosphorylation of IKKα and NF-κB was decreased by rapamycin treatment, and the degradation of IκBα was attenuated. (c) The relative mRNA levels of AST, GDH, GAD, and ODC were decreased by rapamycin treatment. (d) The intracellular contents of AST, GDH, GAD, and ODC were decreased by rapamycin treatment. Protein bands were quantified using Gel-Pro Analyzer 4.0 (Media Cybernetics, Inc., Rockville, MD, USA) (the values represent the , , , ).

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