Research Article

Inhibition of the mTORC1/NF-κB Axis Alters Amino Acid Metabolism in Human Hepatocytes

Figure 2

Raptor silencing impairs mTORC1 signaling and NF-κB activation and decreases the expression of AST, GDH, GAD, and ODC in hepatocytes. (a) The phosphorylation of mTOR and S6 was reduced by Raptor silencing. (b) The phosphorylation of NF-κB was reduced by Raptor silencing. (c) The relative mRNA levels of AST, GDH, GAD, and ODC were decreased by Raptor silencing. (d) The intracellular contents of AST, GDH, GAD, and ODC were decreased by Raptor silencing. Protein bands were quantified using Gel-Pro Analyzer 4.0 (Media Cybernetics, Inc., Rockville, MD, USA) (the values are presented as the , , , ).
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