Inhibition of the mTORC1/NF-κB Axis Alters Amino Acid Metabolism in Human Hepatocytes

Figure 4

SC75741 inhibits NF-κB activation and downregulates the expression levels of AST, GDH, GAD, and ODC in hepatocytes. (a) The phosphorylation of NF-κB was inhibited by SC75741 treatment. (b) The relative mRNA levels of AST, GDH, GAD, and ODC were decreased by SC75741 treatment. (c) The intracellular contents of GDH, GAD, and ODC were decreased by SC75741 treatment. Protein bands were quantified using Gel-Pro Analyzer 4.0 (Media Cybernetics, Inc., Rockville, MD, USA) (the values represent the , , , ).

(a)

(b)

(c)