Research Article

Anti-Inflammatory Effects of Antarctic Lichen Umbilicaria antarctica Methanol Extract in Lipopolysaccharide-Stimulated RAW 264.7 Macrophage Cells and Zebrafish Model

Figure 5

Effect of UA extract on the regulation of the nuclear factor- (NF-) κB pathway. Cells were treated with the indicated concentrations of UA extract for 1 h, followed by induction with LPS (0.5 μg/mL) for 30 min. Equal amounts of protein were analyzed using specific antibodies against p-IκB and p65. Levels of p-IκB and p65 protein in (a) cytosolic and (b) nuclear fractions measured by western blotting. Three independent experiments were performed, and the data are presented as the . and compared to the cells treated with LPS alone. Expressions of GAPDH and PCNA were used as internal controls for western blot analysis.
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