Research Article

Biofilm Formation by Pathogens Causing Ventilator-Associated Pneumonia at Intensive Care Units in a Tertiary Care Hospital: An Armor for Refuge

Table 1

Biochemical tests for identification of gram-negative isolates.

Organism isolatedE. coliK. pneumoniaeC. freundiiC. koseriP. aeruginosaACBCBCC

TSI (slant/butt)A/AA/AKorA/AKorA/AK/KK/KK/K
Gas+++++---
OF (glucose)FFFFOOO
H2S--±----
MotilityMotileNonmotileMotileMotileMotileNonmotileMotile
Indole+--+---
Citrate-++++++
Urease-+±±±±±
Oxidase----+-+
Lysine decarboxylase++----±
Arginine dihydrolase±-±±+±-
Ornithine decarboxylase±-±+--±

Abbreviation: TSI: triple sugar iron agar; OF: oxidation-fermentation test; A: acidic (yellow); K: alkaline (red); ACBC: Acinetobacter calcoaceticus baumannii complex; BCC: Burkholderia cepacia complex; O: oxidative; F: fermentative.