Bilobalide Enhances AMPK Activity to Improve Liver Injury and Metabolic Disorders in STZ-Induced Diabetes in Immature Rats via Regulating HMGB1/TLR4/NF-κB Signaling Pathway

Figure 5

BB modulated hepatic antioxidant in STZ-induced diabetic rats through AMPK activation and HMGB1/TLR4/NF-κB signaling pathway. The one-day-old immature rats were randomly allocated to 5 groups: healthy control, diabetes mellitus model (DM), STZ+BB (10 mg/kg), STZ+AMPK inhibitor (compound C, CC), STZ+BB (10 mg/kg)+CC. (a, b) The expressions of AMPKα1, HMGB1, TLR4, P65, and p-P65 in hepatic tissues were assayed using Western blot analysis. β-Actin is a loading control. (c) The degree of liver damage was identified by H&E staining and Suzuki score. (d–g) Glucose, TG, AST, and ALT levels were determined using commercial kits. (vs. control); ^# (vs. DM); ^## (vs. DM); ^& (vs. DM+BB (10 mg/kg)).

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