Effects of Remimazolam and Propofol on Ca²⁺ Regulation by Ryanodine Receptor 1 with Malignant Hyperthermia Mutation

Table 4

Median effective concentration (EC₅₀) values of caffeine in wild-type or mutant ryanodine receptor 1- (RYR1-) transfected cells perfused with remimazolam or propofol. EC₅₀ values for caffeine in wild-type and all mutant RYR1-transfected cells were not significantly different with or without remimazolam (546 μM) and propofol (100 μM) perfusion.

RYR1	Perfusion solution		(mM)	95% CI (mM)	value
Wild-type	HBSS	30		2.46 to 3.10
		19		2.31 to 3.51	0.764555
		25		2.50 to 3.03	0.829001
p.Thr84Met	HBSS	16		1.49 to 1.95
		16		1.26 to 1.91	0.623386
		16		1.36 to 1.96	0.730406
p.Ser2345Arg	HBSS	22		1.58 to 2.08
		11		1.27 to 2.21	0.472894
		13		1.17 to 1.78	0.209642
p.Ala4894Thr	HBSS	28		1.18 to 1.60
		17		0.92 to 1.48	0.594602
		17		0.82 to 1.38	0.917061

SD: standard deviation; : number of cells examined; 95% CI: 95% confidence interval; values vs. Hank’s balanced salt solution (HBSS) perfusion.