Development of Microsatellite Marker System to Determine the Genetic Diversity of Experimental Chicken, Duck, Goose, and Pigeon Populations

Table 1

Number of alleles, optimal amplification conditions, and fragment length of 29 alleles for the laboratory chickens.

Loci	Primer sequence (5-3)	Temperature(°C)	Allele range	Applicable groups
MCW0029	GTGGACACCCATTTGTACCCTATG	63.8	139-188	Outbred group
	CATGCAATTCAGGACCGTGCA
ADL0293	GTAATCTAGAAACCCCATCT	53.9	106-120	Outbred group
	ACATACCGCAGTCTTTGTTC
ADL0317	AGTTGGTTTCAGCCATCCAT	58.5	177-219	Outbred group
	CCCAGAGCACACTGTCACTG
GCT0016	TCCAAGGTTCTCCAGTTC	52.2	111-148	Outbred group
	GGCATAAGGATAGCAACAG
ADL0304	GGGGAGGAACTCTGGAAATG	53.9	138-161	Outbred group
	CCTCATGCTTCGTGCTTTTT
LEI0074	GACCTGGTCCTGACATGGGTG	58.5	221-243	Outbred group
	GTTTGCTGATTAGCCATCGCG
ADL328	CACCCATAGCTGTGACTTTG	53.9	107-120	Outbred group
	AAAACCGGAATGTGTAACTG
GGANTECl	GCGGGGCCGTTATCAGAGCA	65.0	139-194	Outbred group
	AGTGCAGGGCGCTCCTGGT
LEI094	CAGGATGGCTGTTATGCTTCCA	56.0	176-211	Outbred group
	CACAGTGCAGAGTGGTGCGA
MCW0330	TGGACCTCATCAGTCTGACAG	58.5	217-287	Outbred group
	AATGTTCTCATAGAGTTCCTGC
LEI0141	CGCATTTGATGCATAACACATG	52.2	221-245	Outbred group
	AAGGCAAACTCAGCTGGAACG
MCW0087	ATTTCTGCAGCCAACTTGGAG	58.5	268-289	Outbred group
	CTCAGGCAGTTCTCAAGAACA
MCW0347	GCTTCCAGATGAGCTCCATGG	52.0	121-149	Outbred group
	CACAGCGCTGCAGCAACTG
ADL176	TTGTGGATTCTGGTGGTAGC	58.5	183-200	Outbred group
	TTCTCCCGTAACACTCGTCA
ADL0201	GCTGAGGATTCAGATAAGAC	58.5	111-151	Outbred group
	AATGGCYGACGTTTCACAGC
GGNCAMZO	GTCACTAGGTTAGCAGCATG	56.0	234	Outbred group
	GCTGGATACAGACCTCGATT			Haplotype
GGAVIR	AGAGATGGTGCACGCAACCT	60.7	86-89	Outbred group
	CGAGCACTTTCTGGCAGAGA			Haplotype
MCW0063	GGCTCCAAAAGCTTGTTCTTAGCT	53.9	116-146	Outbred group
	GAAAACCAGTAAAGCTTCTTAC			Haplotype
ADL185	CATGGCAGCTGACTCCAGAT	58.5	116-142	Outbred group
	AGCGTTACCTGTTCGTTTGC			Haplotype
GGMYC	CGAGGCGCTCTGCGAGTTTA	62.4	139-151	Outbred group
	TGGGGACCTCTGGCTCTGAC			Haplotype
LEI0094	GATCTCACCAGTATGAGCTGC	53.9	250-283	Outbred group
	TCTCACACTGTAACACAGTGC			Haplotype
GGVITC	AGCCATCATTCAGGGCATCT	58.5	86	Outbred group
	GATGTCCTGAGTGATGCTCA			Haplotype
ADL0292	CCAAATCAGGCAAAACTTCT	58.5	110-136	Outbred group
	AAATGGCCTAAGGATGAGGA			Haplotype
GGVITIIG	GGCAGGTTTCTAATGCCTGA	56.0	186-189	Outbred group
	CCCATCGTTTCAACTGTATG			Haplotype
ADL166	TGCCAGCCCGTAATCATAGG	58.5	131-154	Outbred group
	AAGCACCACGACCCAATCTA			Haplotype
MCW0014	AAAATATTGGCTCTAGGAACTGTC	58.5	172-195	Outbred group
	ACCGGAAATGAAGGTAAGACTAGC			Haplotype
GGCYMA	AGCGAGGCGCTCTGCGAGTT	64.6	140-153	Outbred group
	GGGCACCTCTGGCTCTGACC			Haplotype
MCW0402	ACTGTGCCTAGGACTAGCTG	56.0	141-229	Outbred group
	CCTAAGTCTGGGCTCTTCTG			Haplotype
STMSGGHU2-1A	CTTAATATGTGTGAGGTGGC	53.9	235-238	Haplotype
	GTTCTCACAATTGCATTAGC