Development of Microsatellite Marker System to Determine the Genetic Diversity of Experimental Chicken, Duck, Goose, and Pigeon Populations

Table 4

Number of alleles, optimal amplification conditions, and fragment length of 16 alleles for the outbred colony laboratory pigeons.

Loci	Primer sequence(5-3)	Temperature (°C)	Allele range
UU-Cli02	TGGGCAAGGTACACTTTTAGGT	61.0	158-170
	CTTTATGCTCCCCCTTGAGAT
UU-Cli06	TTTGAAAAACATGGATTGTGC	56.0	140-145
	AATTTGCAGAGGGTGAGTGG
PG5	GTTCTTGGTGTTGCATGGATGC	59.0	262-266
	AGTTACGAAATGATTGCCAGAAG
C26L9(1265223)	CAAAGCTGCTGACGTGAATCAA	59.0	467-472
	AGAGACGCTCCATGCAAAAG
UU-Cli14	CAGAACGTTTTGTTCTGTTTGG	58.0	265-292
	TCTTGCTGCAGTCTTCATCC
C12L1(532572)	GTTGTTTGGCTGAGTGGACG	62.0	126-136
	TCAACCAGGGGAATTGGCAG
C12L4(906353)	GCTGCTGTCTTCTTCATTGGG	60.0	210-250
	TTAAAACCTCCCGTCTCCCTG
CliμD11	CCAATCCCAAAGAGGATTAT	58.0	78-98
	ACTGTCCTATGGCTGAAGTG
C26L10(1404758)	GCTGTCAGGTATCAGCCACAA	59.0	211-226
	TCAGACCCACGAAAGCTGTAA
C26L4(568923)	CAACCCCATGTGGGTGAGAC	63.0	357-432
	CACCACCACGTGGGACATC
PG4	CCCATCTCCTGCCTGATGC	64.0	136-170
	CACAGCAGGATGCTGCCTGC
UU-Cli12	CGCCAGACTGTATTGTGAGC	61.0	231-265
	AGCATGGCTGTTCTTTGAGG
CliμT47	ATGTGTGTTTGTGCATGAAG	56.0	183-214
	ATGAAAGCCTGTTAGTGGAA
CliμD32	GAGCCATTTCAGTGAGTGACA	60.0	136-158
	GTTTGCAGGAGCGTGTAGAGAAGT
UU-Cli07	GCTGCCTGTTACTACCTGAGC	61.0	277-310
	CTGGCCATGAAATGAACTCC
C26L1(20390)	AGGAGCCTACACTGGGTTTTC	60.0	250-268
	TGTAGCTCTGCAATCAGCCT