Curcumin Targets Both Apoptosis and Necroptosis in Acidity-Tolerant Prostate Carcinoma Cells

Figure 4

Effects of ATP supplementation on curcumin-treated PC-3 and PC-3AcT cells. (a) Cells were pretreated with or without NAC (5 mM) for 2 h prior to treatment with CCM (40 μM) for the indicated times in DMEM containing lactic acid (3.8 μM). Cellular ATP levels were measured by CellTiter-Glo luminescent cell viability assay. Cells were pretreated with ATP (1 mM), 2 h prior to treatment with CCM (40 μM) for 48 h in DMEM containing lactic acid (3.8 μM). Cell viability was measured by MTT assay (b). The levels of DNA damage response- (c) apoptosis-, and necroptosis- (d) mediated proteins were assessed by Western blotting. vs. respective control cells. ^# vs. respective CCM-treated cells. CCM: curcumin; NAC: N-acetylcysteine.

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