Knockdown of MALAT1 Inhibits the Progression of Chronic Periodontitis via Targeting miR-769-5p/HIF3A Axis

Figure 3

miR-769-5p mimics alleviated LPS-induced PDLC injury. (a) The expression of miR-769-5p was detected by qRT-PCR in gingival tissues of patients with CP and healthy controls. vs. healthy controls. (b) The expression of miR-769-5p was detected by qRT-PCR in LPS-treated PDLCs and control cells. vs. control cells. (c) The expression of miR-769-5p was detected by qRT-PCR in PDLCs transfected with miR-NC, miR-769-5p mimics, or miR-769-5p inhibitor. vs. the miR-NC group. (d) Cell viability was detected by MTT assay in LPS-treated PDLCs transfected with miR-NC or miR-769-5p mimics. vs. the miR-NC group. (e–g) The concentrations of IL-6, IL-1β, and TNF-α were measured by ELISA in supernatants of LPS-treated PDLCs transfected with miR-NC or miR-769-5p mimics. vs. the miR-NC group. (h) The protein levels of Bax and Bcl-2 were detected by western blot in LPS-treated PDLCs transfected with miR-NC or miR-769-5p mimics. vs. the miR-NC group. (i) The protein level of caspase-3 was detected by western blot in LPS-treated PDLCs transfected with miR-NC or miR-769-5p mimics. vs. the miR-NC group.

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