LDHA-Mediated Glycolytic Metabolism in Nucleus Pulposus Cells Is a Potential Therapeutic Target for Intervertebral Disc Degeneration

Figure 1

Preoperative lumbar MRI images of patient’s intervertebral disc tissue were classified via Pfirrmann’s grading system. Intervertebral disc tissues scored I-II were defined as normal disc or early stage of IDD, and the discs scored IV-V were defined as advanced stage of IDD (LVF: Pfirrmann I-II, IDD: Pfirrmann IV-V). Red arrows refer to the position of the acquired NP tissue. (a) HE staining (200x) showed that the volume of nucleus pulposus cells in IDD tissues decreased, and the cell spacing was reduced due to tissue shrink age. (b) Immunohistochemical staining (200x) shows the expression of Aggrecan and collagen II expression in the NP tissue from LVF and IDD patients. (c) IF and IHC showed that the expression of SIRT1 in severely degenerated NP cells was significantly reduced. Western blots and quantification data of SIRT1 in NP cells of each group. .  μm.

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