The Potential Genes Mediate the Pathogenicity of Allogeneic CD4⁺T Cell in aGVHD Mouse Model

Figure 5

The verification of the expression of hub genes in vitro. (a) The percent of activated CD4⁺T cells in naive and activated T cells by induction of anti-CD3/CD28 antibodies. (b) The percent of the activated CD4⁺T cells in Balb/c, C57BL/6, and alloactivated T cells after MLR. The activated CD4⁺T cells (CD4⁺CD44⁺) were detected by flow cytometry analysis. (c) The expression levels of genes from activated T cells were detected. (d) The expression levels of genes from FACS-sorted activated CD4⁺T cells were detected. The -axis is the log2 scale of the expression level of hub genes. Each experiment was performed at least three times, and the results were presented as .

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